text: RNA extraction and reverse transcription
  Total RNA were extracted from cells using either Trizol reagent (Invitrogen) or RNeasy kit (Qiagen) and treated with DNaseI reagent (Macherey-Nagel) for 30 min at room temperature. Reverse transcriptions were performed using 1microg total RNA and the High capacity cDNA reverse transcription kit with RNase Inhibitor (Applied Biosystems).
  
entities: ['cells']
entities: Cell
entities: PMC-2922337-sec-10_T1