text: Lactotransferrin binding to its platelet receptor inhibits platelet aggregation.
  A fluorescent lactotransferrin probe was prepared by coupling 5-(([2-(carbhydrazino)methyl]-thio)acetyl)amino fluorescein to aldehyde groups that were produced by a mild periodic-acid oxidation of the glycan moieties of lactotransferrin. In this manner, the receptor-binding site of the lactotransferrin remains active in contrast to the binding site of the lactotransferrin derivatized with fluorescein isothiocyanate. The fluorescent probe allowed us to characterize, by flow cytometry, the binding of lactotransferrin to non-activated human platelets. The putative lactotransferrin platelet receptor was purified and its immunological and physico-chemical properties were found to be very similar to those of the receptor previously isolated from activated human lymphocytes. Lactotransferrin inhibits ADP-induced platelet aggregation at concentrations down to 5 nM, which can be reached in the plasma after leukocyte degranulation. Inhibition of platelet aggregation was also observed with the N-terminal fragment of lactotransferrin (residues 3-281; 50% inhibition = 2 microM) and with CFQWQRNMRKVRGPPVSC synthetic octodecapeptide (residues 20-37; 50% inhibition = 20 microM) corresponding to one of the two external loops (residues 28-34 and 39-42) where we recently located the receptor-binding site. The activity (50% inhibition = 500 microM) of the tetrapeptide KRDS (residues 39-42), which has already been described, was at least 25-times and 16000-times lower than the activity of the octodecapeptide and of the lactotransferrin molecules, respectively. Finally, the inhibition was demonstrated to be mediated by a mechanism which requires the binding of lactotransferrin to its putative receptor and not to platelet glycoprotein IIb-IIIa.
  
entities: ['platelet']
entities: Cell
entities: PMID-8389297_T1
entities: ['platelet']
entities: Cell
entities: PMID-8389297_T2
entities: ['platelets']
entities: Cell
entities: PMID-8389297_T3
entities: ['platelet']
entities: Cell
entities: PMID-8389297_T4
entities: ['lymphocytes']
entities: Cell
entities: PMID-8389297_T5
entities: ['platelet']
entities: Cell
entities: PMID-8389297_T6
entities: ['plasma']
entities: Organism_substance
entities: PMID-8389297_T7
entities: ['leukocyte']
entities: Cell
entities: PMID-8389297_T8
entities: ['platelet']
entities: Cell
entities: PMID-8389297_T9
entities: ['platelet']
entities: Cell
entities: PMID-8389297_T10