passages: PMC-2769146-sec-05__text
passages: sec
passages: 3.2.
  Cell line and culture
  DU145 (prostate cancer) cell line was used in this assay. DU145 was grown in DMEM/F-12 medium (Gibco BRL Life Technologies, San Diego, CA., USA) supplemented with 10% fetal calf serum and kept in a humidified 37 degreesC, 5% CO2 incubator.
  